Which statement best describes the goal of affinity chromatography?

Which statement best describes the goal of affinity chromatography?

• A. All proteins bind to the resin nonspecifically.
• B. Only nonspecific proteins pass, while the target is always ignored.
• C. The protein of interest is degraded during the process.
• D. Everything passes through except the protein of interest; antibodies bind with their complementary antigenic epitope on the protein of interest and pull it out.

Answer: (D)